rabbit anti–human pla2g4a polyclonal antibody (ABclonal Biotechnology)
Structured Review

Rabbit Anti–Human Pla2g4a Polyclonal Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti%E2%80%93human+pla2g4a+polyclonal+antibody/rabbit+anti+human+pla2g4a+polyclonal+antibody/pmc08907092-152-59-63
Average 90 stars, based on 1 article reviews
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1) Product Images from "The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles"
Article Title: The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles
Journal: Reproductive Sciences
doi: 10.1007/s43032-021-00691-3
Figure Legend Snippet: Gene-related information
Techniques Used:
Figure Legend Snippet: Protein expression in CCs and MGCs. Primary antibodies: mouse anti–human ABCA1 polyclonal antibody (Abcam; diluted 1:3000); rabbit anti–human LDLR polyclonal antibody (PTG; diluted 1:1000); rabbit anti–human SCARB1 polyclonal antibody (Abcam; diluted 1:1000); rabbit anti–human SULT2B1 polyclonal antibody (Abclonal; diluted 1:1000); rabbit anti–human CYP19A1 polyclonal antibody (Abclonal; diluted 1:1000); rabbit anti–human CRLS1 polyclonal antibody (PTG; diluted 1:1000); mouse anti–human LPCAT3 polyclonal antibody (Abcam; diluted 1:1000); rabbit anti–human PLA2G4A polyclonal antibody (Abclonal; diluted 1:1000). Secondary antibodies: goat anti-mouse IgG conjugated with horseradish peroxidase (HRP) diluted 1:3000 in TTBS for ABCA1 and LPCAT3 or goat anti–rabbit IgG conjugated with HRP diluted 1:3000 in TTBS for LDLR, SCARB1, SULT2B1, CYP19A1, CRLS1, and PLA2G4A. The expression of mouse anti–human ACTIN polyclonal antibody (Servicebio; diluted 1:3000) was used as an internal reference. A shows the protein expression of ABCA1, LDLR, SCARB1, SULT2B1, and CYP19A1; B shows the protein expression of CRLS1, LPCAT3, and PLA2G4A
Techniques Used: Expressing
Figure Legend Snippet: Gene expression of MGCs and CCs was evaluated by qPCR. A shows the gene expression of ABCA1, ABCG1, LDLR, and SCARB1; B shows the gene expression of SULT2B1, STS, and CYP19A1. In A and B , the gene expression of MGCs is considered to be 1; C shows the gene expression of LPCAT3 and PLA2G4A; D shows the gene expression of PTPMT1 and CRLS1. In C and D , the gene expression of MGCs is considered to be 1. * P < 0.05, ** P < 0.01, Student’s t -test
Techniques Used: Expressing